ABSTRACT
OBJECTIVE@#To investigate the effect of salvianolic acid B on rats with myocardial ischemia-reperfusion injury.@*METHODS@#SD rats were randomly divided into five groups (n=10 in each group): A sham operation group, B ischemic reperfusion group model group, C low dose salvianolic acid B group, D median dose salvianolic acid B group, E high dose salvianolic acid B group. One hour after establishment of the myocardial ischemia-reperfusion model, the concentration and the apoptotic index of the plasma level of myocardial enzymes (CTn I, CK-MB), SOD, MDA, NO, ET were measured. Heart tissues were obtained and micro-structural changes were observed.@*RESULTS@#Compared the model group, the plasma CTn, CK-MB, MDA and ET contents were significantly increased, NO, T-SOD contents were decreased in the treatment group (group C, D, and E) (P<0.05); compared with group E, the plasma CTn I, CK-MB, MDA and ET levels were increased, the NO, T-SOD levels were decreased in groups C and D (P<0.05). Infarct size was significantly reduced, and the myocardial ultrastructural changes were improved significantly in treatment group.@*CONCLUSIONS@#Salvianolic acid B has a significant protective effect on myocardial ischemia-reperfusion injury. It can alleviate oxidative stress, reduce calcium overload, improve endothelial function and so on.
Subject(s)
Animals , Rats , Apoptosis , Benzofurans , Pharmacology , Creatine Kinase, MB Form , Blood , Heart , Myocardial Reperfusion Injury , Drug Therapy , Pathology , Myocardium , Cell Biology , Pathology , Myocytes, Cardiac , Nitric Oxide , Blood , Oxidoreductases , Blood , Protective Agents , Pharmacology , Rats, Sprague-Dawley , Troponin I , BloodABSTRACT
<p><b>OBJECTIVE</b>To optimize the herb processing process for the total contains of psoralen and isopsoralen and the rate of extract in Psoralea corylifolia by micro-wave herb processing.</p><p><b>METHOD</b>The contains of psoralen and isopsoralen was obtained by HPLC. The micro-wave herb processing process was optimized by the way of uniform design and contour map.</p><p><b>RESULT</b>The optimum process was:20% as salt concentration, 4h as immerse time, micro-wave strength as strong, 270 seconds as micro-wave time. The absolute error of the predicted value from the models were smaller than 6% and 0. 3% respectively.</p><p><b>CONCLUSION</b>The regression models are notable and reasonable, which can forecast results precisely.</p>